Influence of alternating temperature preculture on cryopreservation results for potato shoot tips
نویسندگان
چکیده
منابع مشابه
Cryopreservation of Potato Shoot Tips
Potato is one of the most important staple crops in both developed and developing countries of the world. Potato genetic resources are a prerequisite for potato breeding in both conventional and genetic engineering programmes. Cryopreservation has long been considered an ideal means for the long-term conservation of plant genetic resources. This review provides comprehensive information on vari...
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The aim of the present study was to investigate the importance of the preculture conditions on the survival of apple (Malus domestica Borkh., cv. Romus4 and rootstock M106) shoot tips cryopreserved using a combined droplet vitrification procedure. Preculture was critical for the survival of apple shoot tips after cryopreservation. Among the different tested sugars and sugar alcohols (glucose, s...
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Vitrification refers to the physical process by which a liquid supercools to very low temperatures and finally solidifies into a metastable glass, without undergoing crystallization at a practical cooling rate. Thus, vitrification is an effective freeze-avoidance mechanism and living tissue cryopreservation is, in most cases, relying on it. As a glass is exceedingly viscous and stops all chemic...
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Cryopreservation has been recognized as a practical and efficient tool for long-term storage of vegetatively propagated plants. This study was conducted to investigate the effects of sucrose concentration, hardening temperature and different cryopreservation methods on the survival rate of potato shoot tips after cryopreservation. Excised shoot tips of in vitro plantlets of potato cultivars, At...
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BACKGROUND Garlic has lost its ability to form seeds in the course of its domestication. Therefore, the germplasm storage via cryopreservation is increasingly applied. The progression of the various steps within the cryopreservation procedure is accompanied by declining survival rates of the explants. Much of the recent work on cryo-stress has been focussed on osmotic and cold stress components...
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ژورنال
عنوان ژورنال: Plant Cell Reports
سال: 2008
ISSN: 0721-7714,1432-203X
DOI: 10.1007/s00299-008-0574-1