Induction of polyploidy in grapevine (Vitis vinifera L.) seedlings by in vivo colchicineapplications

Polyploidization is an important technique used in grape breeding to create new genetic resources and can be induced using some antimitotic agents, including colchicine. Plants with increased ploidy gain better quality than their original diploids characteristics. In this study, which was carried out obtain polyploid genotypes under vivo conditions, the effects of six (1 g L-1, 2 3 4 5 6 L-1) doses colchicine were applied shoot tip meristem regions seedlings obtained from seeds grapevine cv. Ekşi Kara Trakya İlkeren, for three consecutive days twice a day (at 08.30 18.00 h) investigated. The mutagen evaluated by following treated seedlings? morphological changes comparing them 'Kyoho' (4x) flow cytometry (FC) analysis. surviving plants after treatment, stomatal characteristics differed varying degrees diploids, density decreased inversely increase size genotypes. Among seedlings, 1 tetraploid (5 L-1), İlkeren mixoploid (2 (6 selected characteristics, chloroplast numbers, confirmed FC It determined that effective grapevine, observations, analysis are useful methods obtaining results selection