Induction of haploid plants for speed-up breedıng in sunflower (Helianthus annuus L.) by pollen irradiation

The creation of homozygous plants is essential for both genetic studies and the production hybrid seeds, particularly in that were pollinated by an alien species. Dihaploid (DH) technology important application obtaining lines used to plant speed breeding programs. generation haploid embryos pollen irradiation pollination female flowers with irradiated has been successfully applied. influence on induction haploids sunflower (Helianthus annuus L.) evaluated 16 different from Turkey’s National Sunflower Breeding Program. In this study, grains varying dosages Gamma rays (500-1000 Gy) generate parthenogenetic oilseed sunflowers. At a period 12–20 days following process pollination, plated into modified MS culture media under 16/8 h photoperiod temperature 25◦C. Parthenogenetic 18457 at developmental stages rescued vitro 650 germinated. ploidy analysis regenerants was made chromosome count flow cytometry. Except 500 Gy, all effective inducing plants. Obtained results indicate can be produced K3AD SN:8, IMI 069, 044 genotypes selecting appropriate ray dose embryonic stage. This study focused recover doubled accelerate non-segregating breeding.