Induction of experimental allergic arthritis with outer surface proteins ofborrelia burgdorferi
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چکیده
منابع مشابه
Induction of experimental allergic asthma in cat
19 cats were nper-sensitized to allergic asthma by receiving a concomitant spray ang injection of ovalbumin (10% fresh egg-white in normal saline) every other days for four weeks.then animals were anesthetized and left femoral artery cannulation performed to record atrerial pressure and take blood samples.left femoral vein was cannulated to infuse nutritional fluids and grugs as needed.trachea ...
متن کاملExpression and Purification of Recombinant Outer Surface Protein D of Borrelia burgdorferi
To carry out the immunological experiments on the serum of Multiple Sclerosis (MS) patients, based on a correlation between Borrelia burgdorferi infection and contracting MS autoimmune disease the outer surface protein D (OspD) of the bacterium was expressed and purified. A clone containing the OspD gene in pET11a expression vector under the control of T7 promoter was transformed to the bacteri...
متن کاملExpression of outer surface proteins A and C of Borrelia burgdorferi in Ixodes ricinus.
A total of 472 field-collected Ixodes ricinus ticks from southern Germany were investigated by immunofluorescence for the presence of Borrelia burgdorferi with a polyvalent rabbit immune serum and with monoclonal antibodies specific for outer surface proteins A and C (OspA and OspC, respectively). Borreliae were detected in 90 ticks with the polyvalent immunofluorescence assay. Infection rates ...
متن کاملTemporal regulation of outer surface proteins of the Lyme-disease spirochaete Borrelia burgdorferi.
In the 20 years since the first agent of Lyme disease was discovered, much interest has focused on the possible biological roles of a few outer surface proteins (Osps) in the alternating life cycle that includes ticks and vertebrate hosts. Two major proteins, OspA and OspC, are differentially regulated by the spirochaete Borrelia burgdorferi during the several days when ticks feed. The reciproc...
متن کاملRegulation of expression of major outer surface proteins in Borrelia burgdorferi.
We have characterized a Borrelia burgdorferi clone (CA-11 2A) that does not synthesize the major outer surface proteins, OspA and OspB. While the osp operon is intact and capable of expression, no mRNA transcript is detectable in this clone. These results suggest that osp operon expression in the B. burgdorferi clone can be regulated at the level of transcription.
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ژورنال
عنوان ژورنال: Arthritis & Rheumatism
سال: 1994
ISSN: 0004-3591,1529-0131
DOI: 10.1002/art.1780370713