Inducible Promoter-Repressor System from the Lactobacillus casei Phage φFSW

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Inducible promoter-repressor system from the Lactobacillus casei phage phiFSW.

With the aim to extend the presently available inducible gene expression systems for lactobacilli, we have isolated a thermoinducible promoter-repressor cassette from the temperate Lactobacillus casei phage phiFSW-TI in Escherichia coli. The phiFSW-TI promoter fragment was abutted to the plasmid-borne promoterless beta-glucuronidase (gusA) reporter gene and shown to direct its transcription in ...

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ژورنال

عنوان ژورنال: Applied and Environmental Microbiology

سال: 2002

ISSN: 0099-2240,1098-5336

DOI: 10.1128/aem.68.8.4132-4135.2002