Indexing of Potato leaf roll virus (PLRV) from Potato Growing Areas of Punjab, India
نویسندگان
چکیده
منابع مشابه
Digoxigenin-labelled molecular probe for the simultaneous detection of three potato pathogens: potato spindle tuber viroid (PSTVd), potato virus Y (PVY), and potato leafroll virus (PLRV).
A molecular probe, p3POT, was constructed of PSTVd, PVY, PLRV cDNA fragments introduced into pUC18 vector. Sequencing of the inserts revealed that cloned fragments covered conservative parts of pathogenic genomes. Dot-blot hybridization of digoxigenin-labelled construct to crude extracts from plants infected with different potato viruses proved high sensitivity and specificity of the p3POT prob...
متن کاملتعیین برخی خصوصیات بیولوژیکی و مولکولی potato leafroll virus و potato virus a در استان اصفهان
چکیده سیب زمینی یکی از مهم ترین محصولات زراعی در جهان می باشد. ویروس های متعددی این گیاه را تحت تاثیر قرار می دهند و باعث خسارت اقتصادی قابل توجهی می شوند. ویروس پیچیدگی برگ سیب زمینی potato leafroll virus (plrv) عضو تیپ جنس polerovirus و خانواده luteoviridae و ویروس ای سیب زمینی potato virus a (pva) عضو تیپ جنس potyvirus از خانواده potyviridae شایع ترین ویروس های سیب زمینی می باشند که توسط ش...
15 صفحه اولMolecular Detection of Potato Leaf Roll Polerovirus through Reverse Transcription Polymerase Chain Reaction in Dormant Potato Tubers
A Reverse Transcription-Polymerase Chain Reaction (RT-PCR) technique was applied for the detection of Potato leaf roll polerovirus (PLRV) in dormant potato tubers. A primer pair was designed from the coat protein-encoding fragment of the PLRV genome that amplified a 336-bp product. The amplified product was detected in nucleic acid preparations from leaves and tubers of 5 cultivars and from pur...
متن کاملInfection Pattern of PVY and PLRV in Potato Tubers Obtained from Potato Plants Infected with Both the Viruses
Experiments were conducted to study the plant growth and tuber yield of the seed potato collected from PVY and PLRV infected plants. The seed potatoes were graded as under size (<28 mm), A grade ( 28-40 mm), B grade (41-55 mm) and over size (>55 mm) which were planted following RCBD in the field. Third generation seed potatoes of Diamant variety were used. The incidence of viruses was confirmed...
متن کاملComplete Genome Sequences of Sweet potato feathery mottle virus and Sweet potato virus G from Brazil
In Brazil, Potyvirus species in sweet potatoes have been detected mostly by serology. Here, we report the complete genome sequences of two Potyvirus species, Sweet potato feathery mottle virus strain (SPFMV-UNB-01) and Sweet potato virus G strain (SPVG-UNB-01).
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ژورنال
عنوان ژورنال: International Journal of Virology
سال: 2014
ISSN: 1816-4900
DOI: 10.3923/ijv.2014.272.279