Increased Flux of Lipid Metabolism Enhances Bioethanol Fermentability and Inhibitor Tolerance of Xylose-Utilizing Zymomonas mobilis

The microbial production of fuel ethanol is an attractive and sustainable biotechnological approach. This study presents a metabolic engineering strategy Zymomonas mobilis aimed at coproducing bioethanol fatty acids. increased flux acids stabilizes the cell membrane thus counteracts progressively higher toxicity. In glucose medium, highest titer achieved was 146.7 g/kg broth, surpassing wild-type Z. CP4 angel yeast by 30% 45%, respectively. recombinant strain exhibited total acid 0.4 g/L from 230 sugar solution (5 L bioreactor), representing 12-fold increase compared to CP4. Furthermore, when using 4:2:1 mixture glucose: xylose: mannose (w/v), concentration 142.8 broth attained, only 2.66% lower than that glucose-only medium. These findings highlight enormous potential this genetically engineered for lignocellulosic renewable carbon sources.