Incorporation of radioiodotyroisines into proteins formed during cell-free translation.

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Residue-specific Incorporation of Noncanonical Amino Acids into Model Proteins Using an Escherichia coli Cell-free Transcription-translation System.

The canonical set of amino acids leads to an exceptionally wide range of protein functionality. Nevertheless, the set of residues still imposes limitations on potential protein applications. The incorporation of noncanonical amino acids can enlarge this scope. There are two complementary approaches for the incorporation of noncanonical amino acids. For site-specific incorporation, in addition t...

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On the Significance of Measurements of Amino Acid Incorporation into the Proteins of Cell-free Preparations.

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Posttranslational incorporation of contractile proteins into myofibrils in a cell-free system

The incorporation of newly synthesized protein into myofibrils has been examined in a cell-free system. Myofibrils were added to a reticulocyte lysate after the in vitro translation of muscle-specific poly(A)+RNA. Only a small number of the many synthesized proteins were found to associate with the exogenously added myofibrils. These proteins were all identified as sarcomeric components and had...

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Posttranslational Incorporation of Contractile Proteins into Myofibrils m a Cell-free System

The incorporation of newly synthesized protein into myofibrils has been examined in a cell-free system. Myofibrils were added to a reticulocyte lysate after the in vitro translation of muscle-specific poly(A)+RNA. Only a small number of the many synthesized proteins were found to associate with the exogenously added myofibrils. These proteins were all identified as sarcomeric components and had...

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ژورنال

عنوان ژورنال: Journal of Biological Chemistry

سال: 1978

ISSN: 0021-9258

DOI: 10.1016/s0021-9258(19)62320-3