Incorporation of methionine-derived methyl groups into sirohaem by Escherichia coli
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چکیده
منابع مشابه
Incorporation of L-methionine-methyl-14C into gentamicins.
Gentamicin C complex, produced by Micromonospora purpurea1*2), is composed of three components, Cla, C2 and Q (Fig. 1). These components are methylaminoglycosides, which are distinct from other related antibiotics such as the kanamycins, neomycinsand paromomycins. As seen in Fig. 1, gentamicins Cla, C2, and Q possess one C-methyl and one N-methyl group, two C-methyl and one N-methyl groups and ...
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The methionine analog norleucine was produced during the synthesis of bovine somatotropin by Escherichia coli strain W3110G containing the recombinant plasmid pBGH1. Norleucine was generated by the leucine biosynthetic pathway from pyruvate or alpha-ketobutyrate in place of alpha-ketoisovalerate as the initial substrate. The intracellular level of norleucine was high enough to permit the analog...
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Evidence that ribosomes are an important site of protein synthesis has come mainly from experiments with animal cells. It has been shown in vivo that C14 amino acids are incorporated into peptide linkage first in ribosomes, and by addition of cold amino acids, they can be chased to the bulk of the proteins of the cell.1-3 In vitro, they become bound in ribosomal proteins2 or, after their passag...
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Methyl-14C-gentamicins were prepared from incubation of Micromonospora purpurea in the presence of added methyl-'4C-L-methionine, and products with high specific radioactivity were isolated in satisfactory yields. In the previous paper1), we have shown in shake flask fermentation a high and selective incorporation of radioactivity from methyl-14C-L-methionine into gentamicins. Consequently, we ...
متن کاملReduction of methionine sulfoxide to methionine by Escherichia coli.
L-Methionine-dl-sulfoxide can support the growth of an Escherichia coli methionine auxotroph, suggesting the presence of an enzyme(s) capable of reducing the sulfoxide to methionine. This was verified by showing that a cell-free extract of E. coli catalyzes the conversion of methionine sulfoxide to methionine. This reaction required reduced nicotinamide adenine dinucleotide phosphate and a gene...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1977
ISSN: 0264-6021
DOI: 10.1042/bj1670669