Incorporation of fatty acids into monogalactosyl diglycerides by spinach leaf cell-free preparations

The Incorporation of Amino Acids into Soluble Lipoproteins by Cell-free Preparations from Rat Liver*

The net synthesis of low density plasma lipoproteins has been shown in the perfused rat liver and in rat liver slices in work reported from this laboratory (2). Haft, Roheim, White, and Eder (3) demonstrated amino acid incorporation into low and high density plasma lipoproteins in the perfused liver, and Radding and Steinberg (4) reported similar observations in rat liver slices. The present ex...

Incorporation of Photosensitive Fatty Acids into Phospholipids

In an approach to the study of phospholipid-protein interactions in biological membranes, the photoactivable fatty acids, w-(m-azidophenoxy)-undecanoic acid (I) and o-(m-diazirinophenoxy)-hexadecanoic acid (11), were incorporated biosynthetically into the phospholipids of the Escherichia coli fatty acid auxotroph, strain K1060-B5. The extent of incorportation of the two fatty acids was 43% and ...

Incorporation of radioactive citrate into fatty acids.

BRADY AND GURIN 1 and DITURI AND GURIN 2 were the first to show that soluble enzymes of pigeon liver could synthesize long-chain fatty acids from acetate and that citrate stimulated this process. These results were confirmed and extended to a number of other tissues by other workers and purification of these systems has led to a rather detailed understanding of fatty acid synthesisa, 4. One obs...

C Amino Acid Incorporation by Spinach Chloroplast Preparations.

Biohydrogenation of unsaturated fatty acids. Hydrogenation by cell-free preparations of Butyrivibrio fibrisolvens.

Hydrogenation of cis-9,trans-11-octadecadienoic acid to yield trans-11-octadecenoic acid by cell-free preparations of Butyrivibrio fibrisolvens has been obtained under strictly anaerobic conditions. Reduced methyl viologen, NADH, and an endogenous electron donor each can serve as a reductant. Inhibition studies and gel filtration patterns reveal the presence of at least two hydrogenation system...