Inbreeding, outbreeding and RAPD markers studies of faba bean (Vicia faba L.) crop

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Inbreeding, outbreeding and RAPD markers studies of faba bean (Vicia faba L.) crop

Five faba bean genotypes (Vicia faba L.) were selfed for two cycles to produce S1 and S2 generations. A half-diallel cross was carried out among them in each level of inbreeding (S0, S1 and S2) to obtain 10 F1 hybrids. Parental materials as well as their respective F1s were evaluated during the winter season of 2012. All studied traits except total dry seed yield showed significant inbreeding d...

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Genetic diversity of Palestine landraces of faba bean (Vicia faba) based on RAPD markers.

Until now, neither phenotypic nor molecular approaches have been used to characterize the landraces of Palestine faba beans (Vicia faba). We used PCR-based RAPD markers to determine the genetic diversity and relatedness among 26 Palestinian faba bean landraces (traditional farmers' varieties) from 8 localities in the West Bank, Palestine. In tests with 37 primers, 14 generated no polymorphic ba...

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Enhancing faba bean (Vicia faba L.) genome resources

Grain legume improvement is currently impeded by a lack of genomic resources. The paucity of genome information for faba bean can be attributed to the intrinsic difficulties of assembling/annotating its giant (~13 Gb) genome. In order to address this challenge, RNA-sequencing analysis was performed on faba bean (cv. Wizard) leaves. Read alignment to the faba bean reference transcriptome identif...

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Development of EST-SSR markers and construction of a linkage map in faba bean (Vicia faba)

To develop a high density linkage map in faba bean, a total of 1,363 FBES (Faba bean expressed sequence tag [EST]-derived simple sequence repeat [SSR]) markers were designed based on 5,090 non-redundant ESTs developed in this study. A total of 109 plants of a 'Nubaria 2' × 'Misr 3' F2 mapping population were used for map construction. Because the parents were not pure homozygous lines, the 109 ...

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Gene Function Expression Profile of Faba bean (Vicia faba) Seeds

Faba bean (Vicia faba L) is one of the important grain crops worldwide and its genome, the largest among grain legumes (approx. 13.4 Gb), has yet to be sequenced. Comprehensive knowledge of genes expressed in the crop's large seeds would not only help drive new genetic improvements in the crop but also aid its future genome characterization. Here, we applied high throughput RNASeq (Quantificati...

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ژورنال

عنوان ژورنال: Journal of Advanced Research

سال: 2015

ISSN: 2090-1232

DOI: 10.1016/j.jare.2014.07.004