Inactivation of Pea Leaf Phosphofructokinase by Light and Dithiothreitol
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چکیده
منابع مشابه
Inactivation of pea leaf phosphofructokinase by light and dithiothreitol.
When intact pea (Pisum sativum L.) plants are illuminated, the glycolytic enzyme phosphofructokinase is inactivated. In crude extracts the enzyme is inhibited by dithiothreitol. It would seem that this cytoplasmic enzyme, like glucose-6-P dehydrogenase, is light-inactivated when the enzymes of photosynthetic carbon metabolism are light-activated.
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Chloroplastic phosphofructokinase, phosphorylase, phosphoglucomutase, and phosphoglucoisomerase in peas are light inactivated. The effect of light on phosphofructokinase is mimicked by dithiothreitol. DCMU, arsenite, and sulfite inhibit light modulation of the enzyme. No effect of light inactivation on the K(m) (fructose-6-P) or pH optima of phosphofructokinase was observed.
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1975
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.55.2.199