Inactivation of human norovirus by chlorous acid water, a novel chlorine-based disinfectant

IntroductionHuman norovirus (HuNoV) is a leading cause of infectious gastroenteritis. Since HuNoV shows resistance to alcohol, chlorine-based sanitizers are applied decontaminate the virus on environmental surfaces. Chlorous acid water (CA) has been recently approved as novel disinfectant categorized Type 2 OTC medicine in Japan. In this study, we aimed evaluate capability CA inactivate HuNoV.MethodsHuNoV (genogroups GII.2 and GII.4) was exposed test disinfectants including sodium hypochlorite (NaClO), residual RNA copy measured by reverse transcription quantitative PCR (RT-qPCR) after pretreatment with RNase. addition, log10 reduction number NaClO compared presence bovine serum albumin (BSA), sheep red blood cells (SRBC), polypeptone, meat extract or amino acids stability these under organic-matter-rich conditions.ResultsIn absence organic substances, 200 ppm free available chlorine provided >3.0 within 5 min. Even high matter load (0.3% each BSA SRBC 0.5% polypeptone), achieved while less than 1.0 observed 1,000 (NaClO) polypeptone. reacted only cysteine, histidine glutathione all tested.ConclusionsCA an effective conditions.