In vivo protein crystallization in living insect cells
نویسندگان
چکیده
منابع مشابه
Protein crystallization in vivo
Protein crystallization in vivo provides some fascinating examples of biological selfassembly. Here, we provide a selective survey to show the diversity of functions for which protein crystals are used, and the physical properties of the crystals that are exploited. Where known, we emphasize how the nature of the protein-protein interactions leads to control of the crystallization behaviour.
متن کاملReal-time investigation of dynamic protein crystallization in living cells.
X-ray crystallography requires sufficiently large crystals to obtain structural insights at atomic resolution, routinely obtained in vitro by time-consuming screening. Recently, successful data collection was reported from protein microcrystals grown within living cells using highly brilliant free-electron laser and third-generation synchrotron radiation. Here, we analyzed in vivo crystal growt...
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Background: VP4 protein is as spikes on rotavirus outer capsid shell which is responsible for virus attachment to the host. VP4 induces production of neutralizing antibodies which could be used for serotyping of different isolates. Methods: Simian rotavirus SA11 gene 4 cDNA was cloned into a cloning plasmid pDONRTM by recombination reaction using clonase II enzyme mix. The resulting clone was c...
متن کاملتعیین ارزش غذایی برخی از ضایعات کشاورزی با استفاده از روشهای ,in vitro,in situ in vivo
چکیده ندارد.
15 صفحه اولReal-time investigation of dynamic protein crystallization in living cellsa)
R. Sch€ onherr, M. Klinge, J. M. Rudolph, K. Fita, D. Rehders, F. L€ ubber, S. Schneegans, I. V. Majoul, M. Duszenko, C. Betzel, A. Brandariz-Nu~ nez, J. Martinez-Costas, R. Duden, and L. Redecke Institute of Biology, Center for Structural and Cell Biology in Medicine, University of L€ ubeck, Ratzeburger Allee 160, 23562 L€ ubeck, Germany Institute of Biochemistry, Center for Structural and Cel...
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ژورنال
عنوان ژورنال: Acta Crystallographica Section A Foundations and Advances
سال: 2014
ISSN: 2053-2733
DOI: 10.1107/s2053273314096569