In vivo assessment of salinity stress tolerance in transgenic Arabidopsis plants expressing Solanum tuberosum D200 gene

نویسندگان

چکیده

Transgenic Arabidopsis plants expressing a potato D200 gene encoding hypothetical protein were subjected to salinity stress and assessed for their tolerance. The lines exhibited increased chlorophyll content, improved stomatal conductance, less electrolyte leakage, lower accumulation of malondialdehyde (MDA), higher amount proline compared the wild type (WT) under stress. expression analysis revealed that accumulated significantly mRNA transcripts genes three major antioxidant enzymes ascorbate peroxidase (APX), catalase (CAT), superoxide dismutase (SOD). Chlorophyll fluorescence kinetics analyses showed more efficient in terms primary photochemistry photosystem II performance indices. Furthermore, quantum yields efficiencies represent critical steps photosynthetic light reactions analyzed it was found photosynthetically active than WT salt conditions. Overall, these findings suggest is potential candidate developing stress-resilient crops future.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Assessment of salt tolerance in transgenic tobacco (Nicotiana tobacum L.) plants expressing the AUX gene

Transformation of plants using Agrabacterium rhizogenes may affect secondary metabolite production as well as morphological changes. In this study, T-DNA from Ri plasmid in A. rhizogenes carrying pRi15834-PRT35S-GUS was introduced into tobacco leaf segments to initiate development of transformed hairy roots. Plant regeneration from transgenic roots used MS medium, and plants regenerated fro...

متن کامل

Potato Annexin STANN1 Promotes Drought Tolerance and Mitigates Light Stress in Transgenic Solanum tuberosum L. Plants

Annexins are a family of calcium- and membrane-binding proteins that are important for plant tolerance to adverse environmental conditions. Annexins function to counteract oxidative stress, maintain cell redox homeostasis, and enhance drought tolerance. In the present study, an endogenous annexin, STANN1, was overexpressed to determine whether crop yields could be improved in potato (Solanum tu...

متن کامل

assessment of salt tolerance in transgenic tobacco (nicotiana tobacum l.) plants expressing the aux gene

transformation of plants using agrabacterium rhizogenes may affect secondary metabolite production as well as morphological changes. in this study, t-dna from ri plasmid in a. rhizogenes carrying pri15834-prt35s-gus was introduced into tobacco leaf segments to initiate development of transformed hairy roots. plant regeneration from transgenic roots used ms medium, and plants regenerated fro...

متن کامل

increasing stems length and leaf area in transgenic tobacco plants expressing arabidopsis atexpa18 gene

expansins are cell wall proteins that due to changes in ph, causing the expansion of the cell wall .these proteins are causing deformation of walls through loosening hydrogen bonds between the cellulose and polymer matrix microfibrils .in this study, a root specific gene called atexpa18 -that directly affect the formation of root hairs and also its role  have been proved in other parts of the p...

متن کامل

Improved shoot regeneration protocol for canola explants and pre-assessment of salinity tolerance in canola transgenic plants

Regeneration of explants plays a significant role in plant transformation. Explant type, hormonal concentration, and pre-culturing period are important in transformation efficiency. To get an efficient transformation of canola and optimize regeneration conditions, different explants along with different culture media were studied. Four canola varieties were used to evaluate regeneration ability...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Biologia Plantarum

سال: 2022

ISSN: ['1573-8264', '0006-3134']

DOI: https://doi.org/10.32615/bp.2021.072