In vitro trimerization of OmpF porin secreted by spheroplasts of Escherichia coli.
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چکیده
منابع مشابه
Trimerization of an in vitro synthesized OmpF porin of Escherichia coli outer membrane.
The assembly of outer membrane proteins of Escherichia coli was examined using the OmpF porin as a model. Since this protein is made as a precursor, which is processed to a protein of Mr 37,000 before being assembled into trimers in the outer membrane, we synthesized a modified OmpF, which lacked 16 out of 22 amino acid residues from its signal sequence, in a coupled transcription-translation s...
متن کاملLipopolysaccharide structure required for in vitro trimerization of Escherichia coli OmpF porin.
Deep rought mutants, which produce very defective lipopolysaccharides, are unable to export normal levels of porins into the outer membrane. In this study, we showed that lipopolysaccharides from such mutants were also unable to facilitate the trimerization, in vitro, of monomeric OmpF porin secreted by spheroplasts of Escherichia coli B/r. In contrast, lipopolysaccharides containing most or al...
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Background and Aim: Salmonella Typhi belongs to the family Enterobacteriaceae, gram-negative bacilli and causes gastrointestinal diseases such as typhoid. This bacterium has a special structure and various genes, including the ompf gene (outer membrane protein). Recent studies have shown the possibility of using ompf in the development of a diagnostic tuberculosis vaccine. Therefore, the aim of...
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The in vivo activitty of the recBC nuclease was assayed by transfection of isogenic rec+ and rec-spheroplasts with bacteriophage DNA of various origin and structure. The results indicate that the recBC nuclease can limit transfection at several stages during the production of an infective center; such limitations depend primarily on whether the DNA is in, or assumes, a nuclease-sensitive struct...
متن کاملFlagella of Escherichia coli spheroplasts.
Recently, Z. Vaituzis and R. N. Doetsch (J. Bacteriol. 89:1586, 1965) described the relationship between flagellation and motility in penicillin-induced spheroplasts of Salmonella typhimurium, and they concluded that the formation of flagella was dependent upon prior cell wall synthesis. This conclusion has now been confirmed with Escherichia coli (ATCC 13070), the results found being identical...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1990
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.87.2.743