In-vitro Study of Trachyspermum ammi extract against Diabetes Mellitus

Diabetes resulting from the low secretion of insulin and resistance, is a chronic metabolic disorder. Medication plant materials for treatment Mellitus advised as good source novel drug or leading to preparation drug. We investigated antidiabetic property fractions subfractions obtained ethyl acetate extract Trachyspermum ammi seeds. TLC analysis crude was done. Then column chromatography used isolate T. ammi. Primary screening done by Starch-Agar Gel Diffusion assay identification Alpha-amylase inhibitors. after chromatographic separation subfractions, their secondary carried out Glucose-Uptake in Yeast cells. Finally vitro Anti-diabetic activity screened subfraction evaluated 3T3-L1 cell lines. The results presented along with subfractions. Further line also proved It suggested that had inhibiting α-amylase enzyme, facilitating glucose utilization adipose tissues skeletal muscles.
 HIGHLIGHTS
 
 performed
 inhibitors done
 cells out
 In-vitro lines
 GRAPHICAL ABSTRACT