In vitro Radio Protective Effects of Metformin on Human Lymphocytes Irradiated with Gamma Rays

Radio protective effects of metformin and its ability to alter the spontaneous induced genotoxic cytotoxic levels on human peripheral blood lymphocytes were investigated in this study. Metformin, a hypoglycemic oral drug, is biguanide derived from Galega officinalis that widely utilized controlling type 2 diabetes mellitus. Whole samples 10 healthy donors (5 males 5 females) exposed two doses gamma-rays (1 Gray). Lymphocytes cultures treated with (10 50µM) before gamma-irradiation. Cytokinesis-block micronucleus test was used evaluate radiation genomic damage, cytostasis cytotoxicity, via scoring micronuclei nucleoplasmic bridges once divided binucleated cells as well counting nuclear division index. The results current study revealed increase rate associated decrease index gamma dose dependent manner. Metformin effectively decreased paralleled control increased Moreover, treatment whole 50 µM), h preceding irradiation, remarkably reduced accompanied an rate. introduced be effective radio protector against DNA damage by it can develop materials for protection cancer patients prompted radiotherapy.