In vitro mitochondrial test to assess haemodialyser biocompatibility

In vitro mitochondrial test to assess haemodialyser biocompatibility.

BACKGROUND This paper describes an in vitro mitochondrial test to assess the biocompatibility of haemodialysers. METHODS We tested on isolated liver mitochondria the effect of solutions obtained by an aqueous rinse of different haemodialysers (cuprophane, cellulose acetate, Hemophan, polyacrylonitrile, polymethylmethacrylate, polysulphone, polyamide). Moreover, to determine the penetration in...

Biocompatibility test of light-cured composites in vitro.

The in vitro cytctoxicity to HeLa S3 cells of set specimens of five commercially available light-cured composites was examined by the cell colony-forming method for 6 weeks. Both visible and ultraviolet light-cured composites showed moderate to strong cytotoxicity in the early stage of the experiment, followed by rapid disappearance of cytotoxicity. The light-cured composites showed in vitro bi...

AFM and Cell Staining to Assess the In Vitro Biocompatibility of Opaque Surfaces

Development of a Curved, Stratified, In Vitro Model to Assess Ocular Biocompatibility

PURPOSE To further improve in vitro models of the cornea, this study focused on the creation of a three-dimensional, stratified, curved epithelium; and the subsequent characterization and evaluation of its suitability as a model for biocompatibility testing. METHODS Immortalized human corneal epithelial cells were grown to confluency on curved cellulose filters for seven days, and were then d...

Measuring mitochondrial protein synthesis to assess biogenesis.

IN THEIR PERSPECTIVES ARTICLE, Miller and Hamilton (6) propose that the term “mitochondrial biogenesis” be limited to the measurement of the synthesis rate of mitochondrial proteins. For support, they cite evidence that changes in the abundance of transcription factors for mitochondrial genes or the mRNAs encoding mitochondrial proteins may not result in corresponding changes in mtDNA or protei...