In vitro Maturation, Fertilization and Embryo Development of Immature Buffalo Oocytes Vitrified by Different Cryodevice Types

In Vitro Maturation, Fertilization and Embryo Culture of Oocytes Obtained from Vitrified Auto-Transplanted Mouse Ovary

: the effect of sericin levels (silk glue protein) on rate of in vitro maturation, fertilization and culture of sheep oocytes

هدف از آزمایش اول بررسی اثر سطوح مختلف سریسین [0 (control), 0.1, 0.5, 1.0, 2.5 %] افزوده شده به محیط , ivm بر cumulus cell expansion، بلوغ هسته و توسعه متوالی جنین، در گوسفندان نژاد سنجابی در فصل تولید مثلی می باشد. از سرگیری میوز به وسیله خارج شدن اولین پولار بادی اندازه گیری و هم چنین درصد رسیدن جنین های دو سلولی به مرحله کلیواژ و بلاستوسیت نیز به عنوان نشانه ای از میزان شایستگی توسعه اولیه ج...

Effects of cysteamine during in vitro maturation on viability and meiotic competence of vitrified buffalo oocytes

The aims of the present study were to assess the effects of cysteamine as an anti-oxidant on the rate of in vitro maturation (IVM) of buffalo oocytes (experiment 1), and their viability and nuclear status following vitrification (experiment 2). Immature oocytes with compact cumulus cells obtained from the ovaries of slaughtered animals were harvested and then cultured in the maturation medium w...

In Vitro Maturation, Fertilization and Embryo Culture of Oocytes Obtained from Vitrified Auto-Transplanted Mouse Ovary

BACKGROUND The purpose of this study was to investigate the in vitro survival and developmental potential of oocytes obtained from vitrified mouse ovaries transplanted to a heterotopic site. MATERIALS AND METHODS In this experimental study, two-week-old mice were unilaterally ovariectomized after anesthesia. The ovaries were vitrified by cryotop. After two weeks, the ovaries were thawed and a...

Effect of different types of cryoprotectants on developmental capacity of vitrified-thawed immature buffalo oocytes

Studies were conducted to compare viability of immature buffalo oocytes vitrified in different types of cryoprotectants on the post-thaw morphological appearance, the in vitro maturation and developmental competence of buffalo oocytes. The vitrification solution (VS) consisted of Dulbecco’s phosphate buffered saline (DPBS) supplemented with 0.5 M sucrose, 0.4% bovine serum albumin (BSA) and dif...