In vitro integration of retrotransposon Ty1: a direct physical assay.
نویسندگان
چکیده
منابع مشابه
A ubiquitin-conjugating enzyme, RAD6, affects the distribution of Ty1 retrotransposon integration positions.
A galactose-inducible Ty1 element was used to generate 59 independent Ty1 inserts that inactivate the CAN1 gene. As found in previous studies, the distribution of these elements shows a gradient of insertion frequency from highest to lowest between the 5' and 3' ends of the gene. However, 53 independent Ty1 and Ty2 insertions isolated by an identical procedure in an isogenic rad6 deletion strai...
متن کاملA nucleosomal surface defines an integration hotspot for the Saccharomyces cerevisiae Ty1 retrotransposon.
Ty1, the most abundant retrotransposon in Saccharomyces cerevisiae, integrates preferentially upstream of genes transcribed by RNA polymerase III (Pol III). Targeting is likely due to interactions between the Ty1 integration complex and a feature of chromatin characteristic of sites of Pol III transcription. To better understand Ty1 targeting determinants, >150,000 Ty1 insertions were mapped on...
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Retrotransposons contribute significantly to the size, organization, and genetic diversity of their host genomes. To characterize novel retrotransposon families in pigeonpea and develop retrotransposon-based sequence-specific amplification polymorphic markers, in silico homology sequence search was carried out against the whole genome shotgun sequence of pigeonpea variety Asha (ICPL87119). For ...
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The Ty1 Retrotransposon Restriction Factor p22 Targets Gag
A novel form of copy number control (CNC) helps maintain a low number of Ty1 retrovirus-like transposons in the Saccharomyces genome. Ty1 produces an alternative transcript that encodes p22, a trans-dominant negative inhibitor of Ty1 retrotransposition whose sequence is identical to the C-terminal half of Gag. The level of p22 increases with copy number and inhibits normal Ty1 virus-like partic...
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ژورنال
عنوان ژورنال: Molecular and Cellular Biology
سال: 1994
ISSN: 0270-7306,1098-5549
DOI: 10.1128/mcb.14.9.5719