In Vitro Immature Embryo Culture of Paeonia ostii ‘Feng Dan’

Paeonia ostii ‘Feng Dan’ is an economically important, multipurpose woody plant in terms of its medical, ornamental, and oil values; however, there a noticeable contradiction between the increasing demands lack excellent germplasm resources because traditional breeding propagation approaches. In vitro embryo culture attractive option for this issue. This study presents protocol immature P. Dan’, which involves two steps: 1) seeds at 30 days after anthesis (DAA) (cellularization stage endosperm, proembryo stage) or being cultured cotyledon formation (upward micropyle with placenta was best inoculation method highest ratio seed 66.67%); 2) seedling establishment realized within 7 months via (at 40 DAA after) germination, shoot induction, rooting, acclimatization. The multiplication potential increased maturity. provides available reference rescue tree peony will be beneficial to shortening cycle.