In vitro establishment: Monstera acuminata Koch and Monstera deliciosa Liebm

The study evaluated the aseptic establishment of Monstera acuminata Koch and deliciosa Liebm (Araceae) from leaves induction in vitro organogenesis M. K. stem discs young shoots. For this purpose, different disinfection protocols were applied to mature shoots, which leaf explants approximately 1 cm2 mm thickness extracted. established semi-solid media with hormone treatments during stage organogenesis. Disinfection 3% sodium hypochlorite (NaClO) for 20 min 50% Murashige Skoog[1] medium plant tissue culture preservative (PPM) favored less oxidation both species. All grown PPM-added at survived, showed no contamination more than 80% retained cellular activity up 49 days age. At 35 culture, Tween-20 + 20% ethanol 2.5% NaClO, seeding MS added mg/L BAP, 0.5 AIA 0.1 ANA, seven new shoots induced. was adaptable conditions. Advances native Araceae wicker production are basis ex situ conservation local populations.