In vitro bovine embryo production in a synthetic medium: Embryo development, cryosurvival, and establishment of pregnancy
نویسندگان
چکیده
منابع مشابه
In vitro bovine embryo production in a synthetic medium: embryo development, cryosurvival, and establishment of pregnancy.
The aim of this study was to develop an in vitro embryo culture medium without either fetal calf serum or BSA, using various growth factors and cytokines (GFs-CYKs; IGF-I, IGF-II, bFGF, LIF, GM-CSF, TGF-β1, and PDGF-BB), and other molecules with surfactant and embryotrophic properties, such as recombinant albumin (RA) and hyaluronan (HA). The first part of the study was dedicated to define the ...
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Bovine oocytes were matured, fertilized, and cultured FCM 199 with serum and co-culture) in vitro (IVMFC) with addition, during different phases of the procedure, of antioxidants: superoxide dismutase (SOD) and reduced glutathione (GSH). The addition of SOD (1,500 or 3,000 IU/ml) did not improve proportions of oocytes undergoing cleavage or the development of embryos to morula and blastocyst st...
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Oxidative modifications of cell components due to the action of reactive oxygen species (ROS) is one of the most potentially damaging processes for proper cell function. However, in the last few years it has been observed that ROS participate in physiological processes. The aim of this work was to determine ROS generation during in vitro production of bovine embryos. Cumulus-oocyte complexes we...
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ژورنال
عنوان ژورنال: Theriogenology
سال: 2015
ISSN: 0093-691X
DOI: 10.1016/j.theriogenology.2015.04.014