In vitro activity of methylene blue on Mycobacterium tuberculosis complex isolates

Methylene blue is used for bacterial staining in microbiology and as an antidote drug medicine. In this study, we aimed to investigate the antimicrobial effects of methylene against Mycobacterium tuberculosis complex clinical isolates. Seventeen stored M. isolates were included study. The inoculated into Mycobacteria Growth Indicator Tubes incubated Automated Mycobacterial Detection System. tubes containing at critical concentrations 0.2, 2, 20, 1000 µg ml-1 control tube prepared. Antimicrobial susceptibility testing was performed using System which gold standard second line anti-tuberculosis testing. At end six susceptible all concentrations. Five only blue. Three 20 Susceptibility rate found 94% when proportion accepted 400 GU (1/100 control). Significant relationship observed between administered survival statistical analysis. We conclude that may become a potential agent due its well-known side dosing regimens.