In-Tube DNA Methylation Profiling by Fluorescence Melting Curve Analysis
نویسندگان
چکیده
منابع مشابه
In-tube DNA methylation profiling by fluorescence melting curve analysis.
BACKGROUND Most PCR assays for detection of 5-methylcytosine in genomic DNA entail a two-step procedure, comprising initial PCR amplification and subsequent product analysis in separate operations that usually require manual transfer. These methods generally provide information about methylation of only a few CpG dinucleotides within the target sequence. METHODS An in-tube methylation assay i...
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ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 2001
ISSN: 0009-9147,1530-8561
DOI: 10.1093/clinchem/47.7.1183