In situ metabolic flux analysis to quantify the liver metabolic response to experimental burn injury

Effect of Fasting on the Metabolic Response of Liver to Experimental Burn Injury

Liver metabolism is altered after systemic injuries such as burns and trauma. These changes have been elucidated in rat models of experimental burn injury where the liver was isolated and perfused ex vivo. Because these studies were performed in fasted animals to deplete glycogen stores, thus simplifying quantification of gluconeogenesis, these observations reflect the combined impact of fastin...

C metabolic flux analysis shows that resistin impairs the metabolic response to insulin in L6E9 myotubes

Background: It has been suggested that the adipokine resistin links obesity and insulin resistance, although how resistin acts on muscle metabolism is controversial. We aimed to quantitatively analyse the effects of resistin on the glucose metabolic flux profile and on insulin response in L6E9 myotubes at the metabolic level using a tracer-based metabolomic approach and our in-house developed s...

Manipulating the metabolic response to injury.

In this short review we will concentrate on just one of the features of the metabolic response to injury (classified as accidental trauma, injury or sepsis) which are collectively known as the 'flow' phase. These include an increase in energy expenditure (hypermetabolism), changes in substrate utilisation (insulin resistance) and the focus of this chapter muscle wasting or catabolism. It is rec...

Metabolic response to starvation, injury and sepsis

Synergy between C-metabolic flux analysis and flux balance analysis for understanding metabolic adaption to anaerobiosis in E. coli

Genome-based Flux Balance Analysis (FBA) and steady-state isotopic-labeling-based Metabolic Flux Analysis (MFA) are complimentary approaches to predicting andmeasuring the operation and regulation ofmetabolic networks. Here, genome-derivedmodels of Escherichia coli (E. coli)metabolismwere used for FBA and C-MFA analyses of aerobic and anaerobic growths of wild-type E. coli (K-12 MG1655) cells. ...