In silico study of columbin from Tinospora crispa L as dihydrofolate reductase-thymidylate synthase (DHFR-TS) inhibitor

The Brotowali plant is used to treat malaria. Tinospora crispa L contains active compounds that are good for health and has been widely medicine. Crude extract from can be as target dihydrofolate reductase-thymidylate synthase (DHFR-TS). This research study was analyse the potential chemical content of in form columbine a focus malaria therapy through inhibition In silico method, (CID: 188289) ligand obtained Pubchem while DHFR-TS (PDB ID 2bl9) protein Protein Data Bank, ligands proteins were interacted using HEX 8.0.0.0 visualised discovery studio. results six amino acid residues bind protein. binding an impact on work function Physicochemical analysis shows acts donor acceptor so formed becomes very strong. interaction also supported by act supports forming Van der Waals forces outside hydrogen bonding forces, pi-Alkyl Pi provide support order increase strength stabilisation the. Based discussion, it concluded treatment DHFR-TS.Tanaman digunakan untuk mengobati penyakit mengandung senyawa aktif yang baik kesehatan dan telah banyak pengobatan. Ekstrak kasar dari dapat sebagai Penelitian ini bertujuan menganalisis potensi kandungan kimia berupa kolumbin fokus terapi melalui penghambatan Metode penelitian diperoleh ligan sedangkan diinteraksikan menggunakan divisualisasikan Hasil adalah enam residu asam berikatan dengan DHFR-TS. Pengikatan berdampak pada fungsi kerja Analisis fisikokimia menunjukkan bahwa berperan akseptor sehingga terbentuk menjadi sangat kuat. Interaksi juga didukung oleh bertindak pendukung membentuk gaya di luar ikatan hidrogen Van, pi-Alkil memberikan dukungan dalam rangka meningkatkan kekuatan stabilisasi. Berdasarkan pembahasan disimpulkan berpotensi pengobatan