In Silico Modeling and Characterization of Squalene Synthase and Botryococcene Synthase Enzymes from a Green Photosynthetic Microalga Botryococcus braunii
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چکیده
منابع مشابه
Molecular characterization of squalene synthase from the green microalga Botryococcus braunii, race B.
The green microalga Botryococcus braunii produces large amounts of liquid hydrocarbons and is classified into three races, depending on the type of the hydrocarbon produced. The B race produces two types of triterpenoid hydrocarbons, squalene and botryococcene, both of which are putative condensation products of farnesyl diphosphate. In an attempt to better understand the regulation involved in...
متن کاملTetraterpene Synthase Substrate and Product Specificity in the Green Microalga Botryococcus braunii Race L.
Recently, the biosynthetic pathway for lycopadiene, a C40 tetraterpenoid hydrocarbon, was deciphered from the L race of Botryococcus braunii, an alga that produces hydrocarbon oils capable of being converted into combustible fuels. The lycopadiene pathway is initiated by the squalene synthase (SS)-like enzyme lycopaoctaene synthase (LOS), which catalyzes the head-to-head condensation of two C20...
متن کاملA squalene synthase-like enzyme initiates production of tetraterpenoid hydrocarbons in Botryococcus braunii Race L
The green microalga Botryococcus braunii is considered a promising biofuel feedstock producer due to its prodigious accumulation of hydrocarbon oils that can be converted into fuels. B. braunii Race L produces the C40 tetraterpenoid hydrocarbon lycopadiene via an uncharacterized biosynthetic pathway. Structural similarities suggest this pathway follows a biosynthetic mechanism analogous to that...
متن کاملIdentification of unique mechanisms for triterpene biosynthesis in Botryococcus braunii.
Botryococcene biosynthesis is thought to resemble that of squalene, a metabolite essential for sterol metabolism in all eukaryotes. Squalene arises from an initial condensation of two molecules of farnesyl diphosphate (FPP) to form presqualene diphosphate (PSPP), which then undergoes a reductive rearrangement to form squalene. In principle, botryococcene could arise from an alternative rearrang...
متن کاملIsolation and Characterization of Two Squalene Epoxidase Genes from Botryococcus braunii, Race B
The B race of the green microalga Botryococcus braunii produces triterpene hydrocarbons, botryococcenes and methylsqualenes that can be processed into jet fuels with high heating values. In this alga, squalene is also converted into membrane sterols after 2,3-epoxidation. In the present study, cDNA clones of two distinct squalene epoxidases (BbSQE-I and -II) were isolated. Predicted amino acid ...
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ژورنال
عنوان ژورنال: Journal of Petroleum & Environmental Biotechnology
سال: 2018
ISSN: 2157-7463
DOI: 10.4172/2157-7463.1000371