In-house polymerase chain reaction for affordable and sustainable Chlamydia trachomatis detection in Trinidad and Tobago
نویسندگان
چکیده
منابع مشابه
In-house polymerase chain reaction for affordable and sustainable Chlamydia trachomatis detection in Trinidad and Tobago.
OBJECTIVES To provide a preliminary assessment of in-house polymerase chain reaction (PCR) as an alternative to the more costly commercial test for detection of asymptomatic infection by Chlamydia trachomatis and to provide much needed demographic data on infection indicators within the Trinidad and Tobago public health care system. METHODS An inexpensive in-house nested-PCR with an Internal ...
متن کاملDetection of Chlamydia trachomatis Using polymerase chain reaction (PCR)
Chlamydia trachomatis now is one of the most prevalent bacteria sexually transmissible diseases (STD), and as such, constitutes a serious public health problem. Diagnosis of Chlamydial infections is based on isolation of bacteria in tissue culture media that requires at least 48 to 72h. The aim of this study was the detection of Chlamydia trachomatis, using polymerase chain reaction (PCR), beca...
متن کاملComparison of polymerase chain reaction and bacterial culture for Salmonella detection in the Muscovy duck in Trinidad and Tobago.
OBJECTIVES The purpose of this study was to investigate the presence and serovar identity of Salmonella, at the national level, in farmed Muscovy ducks (Cairina moschata) in Trinidad and Tobago, and to compare the relative benefits of bacterial culture to those of polymerase chain reaction (PCR) for use in the routine detection and surveillance of Salmonella in these ducks. METHODS From March...
متن کاملOptimised sample DNA preparation for detection of Chlamydia trachomatis in synovial tissue by polymerase chain reaction and ligase chain reaction.
OBJECTIVE Molecular biology techniques such as polymerase chain reaction (PCR) and ligase chain reaction (LCR) are routinely used in research for detection of C trachomatis DNA in synovial samples, and these methods are now in use in some clinical laboratories. This study aimed at determining the method best suited to molecular diagnosis of C trachomatis by examining four standard DNA preparati...
متن کاملDetection of Chlamydia trachomatis in urine samples by polymerase chain reaction and enzyme immunoassay.
First-void urine samples collected from sexually transmitted diseases (STD) clinic patients were examined by a nested polymerase chain reaction (PCR) and a commercial enzyme immunoassay (IDEIA Chlamydia) for the diagnosis of Chlamydia trachomatis urethritis or cervicitis. The primers for the PCR amplified a target in the major outer membrane protein (MOMP) gene in C trachomatis while the IDEIA ...
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ژورنال
عنوان ژورنال: Revista Panamericana de Salud Pública
سال: 2007
ISSN: 1020-4989
DOI: 10.1590/s1020-49892007001000004