In-cell RNA structure probing with SHAPE-MaP
نویسندگان
چکیده
منابع مشابه
SHAPE Selection (SHAPES) enrich for RNA structure signal in SHAPE sequencing-based probing data.
Selective 2' Hydroxyl Acylation analyzed by Primer Extension (SHAPE) is an accurate method for probing of RNA secondary structure. In existing SHAPE methods, the SHAPE probing signal is normalized to a no-reagent control to correct for the background caused by premature termination of the reverse transcriptase. Here, we introduce a SHAPE Selection (SHAPES) reagent, N-propanone isatoic anhydride...
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ژورنال
عنوان ژورنال: Nature Protocols
سال: 2018
ISSN: 1754-2189,1750-2799
DOI: 10.1038/nprot.2018.010