Improving Signal/Noise Resolution in Single-Molecule Experiments Using Molecular Constructs with Short Handles
نویسندگان
چکیده
منابع مشابه
Improving signal/noise resolution in single-molecule experiments using molecular constructs with short handles.
We investigate unfolding/folding force kinetics in DNA hairpins exhibiting two and three states with newly designed short dsDNA handles (29 bp) using optical tweezers. We show how the higher stiffness of the molecular setup moderately enhances the signal/noise ratio (SNR) in hopping experiments as compared to conventional long-handled constructs (≅700 bp). The shorter construct results in a sig...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2011
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2011.01.071