Improving lactic acid production via bio-augmentation with acid-tolerant isolates from source-sorted organic household waste

Biotransformation of source-sorted organic household waste (SSOHW) into lactic acid (LA) could be an attractive management approach, converting residual resources to the useful platform chemical and at same time minimizing pollution. However, nutritional content is not always optimal for inoculated bacteria (LAB) due complex composition SSOHW. In present study, autochthonous acid-tolerant LA strains were isolated from The used as bio-augmentation inoculum improving production More specifically, four isolates identified by sequencing Lactobacillus panis DTUAT 01, pontis 02, oris 03, reuteri 04, which efficiently convert glucose pH 4. Kinetic modeling was applied predict performances characteristics isolates, multi-response nonlinear regression analysis performed on experimental data determine kinetic parameters. estimated parameters indicated that strain L. had highest maximum growth rate (µmax) yield among along with relatively high substrate product tolerance. Finally, boosted concentration 29% compared abiotic augmentation (i.e., control test). Likewise, quickly adapt SSOHW shown elimination its lag phase. findings this work can contribute improvement fermentative wastes acidic conditions.