Improving floral nectar storage on filter paper for sugar recovery

Nectar analysis has been used to understand pollination systems, but nectar storage methods have rarely considered as potential sources of inaccuracy in the recovery data. Prompt sugar is not always possible and can affect results. We aimed develop an effective method store on filter paper. Nectars from two subspecies Eremophila maculata (Scrophulariaceae) Strelitzia reginae (Strelitziaceae) were spotted papers. redissolved assayed by high-performance liquid chromatography determine masses sugars recovered papers Day 0 30. evaluated effects elution, paper type size, treatments recovery. Liquid nectars also stored refrigerator. Sugars best eluted 15 mL water agitated for 1 min. small tended be more successfully than those larger (significantly glucose). Paper performed better nylon glucose. Desiccant had a marginal positive effect recovery, did refrigeration storage. If highly accurate measurements are needed, should with large volumes desiccant within few days collection.