Improving Adventitious Shoot Regeneration and Transient Agrobacterium-Mediated Transformation of Apricot (Prunus armeniaca L.) Hypocotyl Sections

The improvement of previously described protocols for the regeneration shoots from ‘Canino’ mature seed hypocotyl slices has been accomplished. effects different factors such as part used, vacuum-infiltration, 2,4-Dichlorophenoxyacetic acid pulse, vacuum-infiltration and sonication on transient transformation were analyzed. When three obtained hypocotyls evaluated separately medium, highest percentages regenerating explants achieved in close to epicotyl central part. On other hand, 30 s followed by during Agrobacterium infection 20 min allowed an increase events. application these modifications procedure increased efficiencies, events may reduce frequency failed experiments. An efficient regeneration/transformation protocol could facilitate its use a biotechnological technique apricot breeding.