Improvement of biomass production by Lactobacillus reuteri using double-carbon source cultivation strategy

Gastric ulcer is caused by Helicobacter pylori which also can cause peptic and gastric cancer. The rise in the global prevalence of antibiotic resistance leads to a continuous decrease eradication rate H. infection. Recently, probiotic treatment, especially Lactobacillus reuteri supplementation has proven show inhibitory effects on able reduce antibiotic-associated side effects. Yet, efficacy treatment hampered lower cell mass production, mainly due low efficiency fermentation process. In this study, cultivation medium was optimized combining two different carbon-sources enhance multiplication. Cultivation media components were conventional statistical methods increase mass. optimization, Plackett-Burman design used screen significantly contributing factors their composition then using response surface methodology. contained (in g L-1): lactose, 30.0; glucose, 45.15; yeast extract, 33.0; K2HPO4, 2.0; CH3COONa, 3.0; C6H17N3O7, MgSO4, 0.5 MnSO4, 0.05. After culturing medium, biomass L. produced 4.12 L−1 compared 1.27 sole carbon-source MRS medium. addition, highly efficient production process developed batch bioreactor system. double-carbon source had improved fermentation.