Improved strategy for mutation detection—a modification to the enzyme mismatch cleavage method
نویسندگان
چکیده
منابع مشابه
Modification of the enzyme mismatch cleavage method using T7 endonuclease I and silver staining.
BENCHMARKS The enzyme mismatch cleavage (EMC) method for mutation detection uses a bacteriophage resolvase enzyme to cleave nonlinear DNA structures such as those caused by the presence of single base mismatches, insertions, and deletions (1). T4 endonuclease VII has been used with great success in several studies, and more recently T7 en-has been shown to have similar properties (2). The advan...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1995
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/23.24.5082