Improved siRNA/shRNA Functionality by Mismatched Duplex
نویسندگان
چکیده
منابع مشابه
Improved siRNA/shRNA Functionality by Mismatched Duplex
siRNA (small interfering RNA) and shRNA (small hairpin RNA) are powerful and commonly used tools in biomedical research. Currently, siRNAs are generally designed as two 21 nt strands of RNA that include a 19 nt completely complementary part and a 2 nt overhang. However, since the si/shRNAs use the endogenous miRNA machinery for gene silencing and the miRNAs are generally 22 nt in length and con...
متن کاملImproved Synthesis of Clifford+T Quantum Functionality
The Clifford+T library provides robust and fault-tolerant realizations for quantum computations. Consequently, (logic) synthesis of Clifford+T quantum circuits became an important research problem. However, previously proposed solutions are either only applicable to very small quantum systems or lead to circuits that are far from being optimal— mainly caused by a local, i.e. column-wise, consid...
متن کاملImproved Mmse-based Multi-user Detectors for Mismatched Delay Channels
Early work on the design of multiuser receivers for Direct-Sequence Code-Division Multiple-Access signals often assumed that any necessary communication parameters were completely known. Motivated by the fact that communication parameters in a practical system need to be estimated, the eeects of parameter mismatch on the performance of Minimum-Mean Squared-Error (MMSE) based multiuser receivers...
متن کاملReactivity of ferrocenylcarbodiimide with DNA duplex containing single-mismatched base pairs.
Ferrocenylcarbodiimide (1), which is known to react with a guanine (G) or thymine (T) base of single stranded DNA, was allowed to react with DNA duplex having a single mismatched base pair of G-T, T-T, or T-cytosine (C). Electrophoreograms of the reaction mixture showed that 1 could react with G or T base of the mismatched sites on the DNA duplex. However, 1 also reacted with the G base of the ...
متن کاملMismatched pyrrolo-dC-modified duplex DNA as a novel probe for sensitive detection of silver ions.
A new technology that enables the highly selective and sensitive detection of silver ions has been developed. The method takes advantage of the unique fluorescence property of a mismatched pyrrolo-dC (PdC)-modified duplex DNA, which serves as the key detection component, and the specific interaction of this duplex with silver ions.
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: PLoS ONE
سال: 2011
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0028580