Improved Mass Defect Model for Theoretical Tryptic Peptides
نویسندگان
چکیده
منابع مشابه
1764 Identification of Tryptic Peptides
Several type C RNA-transforming viruses have been shown to contain acquired cellular sequences within preexisting viral genes (I-3). In many instances expression of proteins encoded by such sequences occurs by inphase read through of translation initiated at the 5' terminus of the viral genome. Resulting polyprotein translational products contain type C viral gag gene amino terminal structural ...
متن کاملPurification of tryptic peptides for mass spectrometry using polyvinylidene fluoride membrane.
A simple procedure for the purification of tryptic peptides, prior to mass spectrometric analysis, using polyvinylidene fluoride membrane (PVDF) is described. The sensitivity of mass spectrometric analysis is such that minor impurities in tryptic peptide digests suppress the signal obtained. However, we obtained useful signal, from a sample that did not yield any spectra earlier, by purifying t...
متن کاملTryptic digestion of ubiquitin standards reveals an improved strategy for identifying ubiquitinated proteins by mass spectrometry.
Ubiquitination plays an essential role in maintaining cellular homeostasis by regulating a multitude of essential processes. The ability to identify ubiquitinated proteins by MS currently relies on a strategy in which ubiquitinated peptides are identified by a 114.1 Da diglycine (GG) tag on lysine residues, which is derived from the C-terminus of ubiquitin, following trypsin digestion. In the f...
متن کاملStatistical characterization of ion trap tandem mass spectra from doubly charged tryptic peptides.
Collision-induced dissociation (CID) is a common ion activation technique used to energize mass-selected peptide ions during tandem mass spectrometry. Characteristic fragment ions form from the cleavage of amide bonds within a peptide undergoing CID, allowing the inference of its amino acid sequence. The statistical characterization of these fragment ions is essential for improving peptide iden...
متن کاملComposition and mass of peptides released during tryptic and chymotryptic hydrolysis of myosin.
Peptides released during the early stages of digestion of myosin by trypsin and chymotrypsin have been isolated and their amino acid compositions determined as a function of the extent of proteolysis. The fractions of the myosin mass released as low molecular weight peptides at various times of digestion are reported. A comparison of the composition of the peptides released by both enzymes indi...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Analytical Chemistry
سال: 2012
ISSN: 0003-2700,1520-6882
DOI: 10.1021/ac203255e