Improved Mammalian Mitochondrial RNA Isolation

RNA Isolation (Small RNA Extraction). Fatemeh Mehrpooyan*

RNA-based phylogenetic methods: application to mammalian mitochondrial RNA sequences.

The PHASE software package allows phylogenetic tree construction with a number of evolutionary models designed specifically for use with RNA sequences that have conserved secondary structure. Evolution in the paired regions of RNAs occurs via compensatory substitutions, hence changes on either side of a pair are correlated. Accounting for this correlation is important for phylogenetic inference...

Release, identification, and isolation of messenger RNA from mammalian ribosomes.

The puromycin-induced dissociation, at high ionic strength, of active ribosomes from reticulocytes resulted in the release of protein-free, apparently undegraded, messenger RNA for globin, which was identified by centrifugation on a sucrose density gradient. This procedure should make messenger RNA from various cells available for isolation on a large scale, and has several advantages over proc...

The post-transcriptional life of mammalian mitochondrial RNA.

Mammalian mitochondria contain their own genome that encodes mRNAs for thirteen essential subunits of the complexes performing oxidative phosphorylation as well as the RNA components (two rRNAs and 22 tRNAs) needed for their translation in mitochondria. All RNA species are produced from single polycistronic precursor RNAs, yet the relative concentrations of various RNAs differ significantly. Th...

Magnetic nanoparticles: an improved method for mitochondrial isolation.

The ultrastructure, function, and physical and chemical properties of mitochondria have become important issues in scientific research. Current mitochondrial isolation methods mainly rely on the physical and chemical properties of mitochondria. Our team presents a fast and reliable new isolation method based on magnetic nanoparticles binding with ...