Improved library preparation with the new iCLIP2 protocol

نویسندگان
چکیده

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

A New Cecal Slurry Preparation Protocol with Improved Long-Term Reproducibility for Animal Models of Sepsis

Sepsis, a life-threatening systemic inflammatory response syndrome induced by infection, is widely studied using laboratory animal models. While cecal-ligation and puncture (CLP) is considered the gold standard model for sepsis research, it may not be preferable for experiments comparing animals of different size or under different dietary regimens. By comparing cecum size, shape, and cecal con...

متن کامل

A strand-specific library preparation protocol for RNA sequencing.

The analysis of transcriptome, which was over the past decade based mostly on microarray technologies, is now being superseded by so-called next generation sequencing (NGS) systems that changed the way to explore entire transcriptome. RNA sequencing (RNA-Seq), one application of NGS, is a powerful tool, providing information not only about the expression level of genes but also further about th...

متن کامل

SENSE: a fast RNA-seq library preparation protocol with superior strand specificity

Introduction With the rapid development of next-generation sequencing technologies, RNA-seq has become the method of choice for transcriptome analysis. Most next-generation sequencing platforms require library construction prior to sequencing, and several current mRNA-seq library preparation protocols conserve the strand orientation of transcripts. This allows the specific assignment of reads t...

متن کامل

Gamifying the library services: A new concept in interacting with users

Background and Aim: Gamification refers to the use of game elements in non-game contexts to motivate engagement. This definition is the most comprehensive one presented so far. The term was proposed first in 2002 but it has been prolonged 8 years to attract individuals’’ attention. Gamification has been applied in various contexts according to its capabilities. The current study aims to investi...

متن کامل

Improved workflows for high throughput library preparation using the transposome-based nextera system

BACKGROUND The Nextera protocol, which utilises a transposome based approach to create libraries for Illumina sequencing, requires pure DNA template, an accurate assessment of input concentration and a column clean-up that limits its applicability for high-throughput sample preparation. We addressed the identified limitations to develop a robust workflow that supports both rapid and high-throug...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Methods

سال: 2020

ISSN: 1046-2023

DOI: 10.1016/j.ymeth.2019.10.003