Improved contiguity of the threespine stickleback genome using long-read sequencing
نویسندگان
چکیده
Abstract While the cost and time for assembling a genome has drastically decreased, it still remains challenge to assemble highly contiguous genome. These challenges are rapidly being overcome by integration of long-read sequencing technologies. Here, we use improve contiguity threespine stickleback fish (Gasterosteus aculeatus) genome, prominent genetic model species. Using Pacific Biosciences sequencing, assembled freshwater from Paxton Lake. contigs this were able fill over 76.7% gaps in existing reference assembly, improving fivefold. Our gap filling approach was accurate, validated 10X Genomics long-distance linked-reads. In addition closing majority gaps, segments telomeres centromeres throughout This highlights power using long reads repetitive difficult regions genomes. latest build been released through newly designed community browser that aims consolidate growing number genomics datasets available fish.
منابع مشابه
An improved assembly of the loblolly pine mega-genome using long-read single-molecule sequencing
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The 22-gigabase genome of loblolly pine (Pinus taeda) is one of the largest ever sequenced. The draft assembly published in 2014 was built entirely from short Illumina reads, with lengths ranging from 100 to 250 base pairs (bp). The assembly was quite fragmented, containing over 11 million contigs whose weighted average (N50) size was 8206 bp. To improve this result, we generated approximately ...
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ژورنال
عنوان ژورنال: G3: Genes, Genomes, Genetics
سال: 2021
ISSN: ['2160-1836']
DOI: https://doi.org/10.1093/g3journal/jkab007