Implications of Bacterial Adaptation to Phenol Degradation under Suboptimal Culture Conditions Involving Stenotrophomonas maltophilia KB2 and Pseudomonas moorei KB4

Despite the well-described abundance of phenol-degrading bacteria, knowledge concerning their degradation abilities under suboptimal conditions is still very limited and needs to be expanded. Therefore, this work aimed study growth potential Stenotrophomonas maltophilia KB2 Pseudomonas moorei KB4 strains toward phenol temperatures, pH, salinity in connection with activity catechol dioxygenases, fatty acid profiling, membrane permeability. The methodology used included: batch culture bacteria minimal medium supplemented (300 mg/L), isolating measuring 1,2- 2,3-dioxygenases, calculating kinetic parameters, chromatographic analysis methyl esters (FAMEs) determining It was established that time utilisation by both high temperatures (39 40 °C) proceeded 10 h; however, at lowest temperature (10 °C), it extended 72 h. P. more sensitive pH (6.5 8.5) than S. degraded 5–6 h longer. Salinity also influenced removal. presence 2.5% NaCl within 28 h, while during ability degrade coupled a relatively and/or 2,3-dioxygenases. FAME profiling permeability measurements indicated crucial alterations bacterial properties leading predominantly an increase saturation obtained results offer hope for use environmental microbiology biotechnology applications.