Impact of galacto-oligosaccharides on prebiotic potential in the intestinal microbiota fermentation and health status in an animal model

The present study was conducted to assess selected galacto-oligosaccharides (GOS) effects on short-chain acids (SCFA), microbiota variability in vitro, and health benefits using an animal model. In the in-vitro anaerobic batch, fermentation applied different groups divided by a varied amount of GOS sources, mixtures, prebiotics. Results reported that SCFA for inulin contributed significantly higher acetic, propionic, butyric acids, resistant starch (RS) showed non-significant effect acetic propionic whereas combined RS values parameters. For bacterial enumeration bifidobacteria compared individual GOS, synergistic were documented. Sprague-Dawley rats given under western diet influence relative high-fat alone observed after 1 4 weeks documented significant levels acid production, body organ weights cecum tend increase dietary intervention (p<0.05). Microbiome data gene sequencing revealed proportion firmicutes lower Bacteroides control rats, which means Lachnospiraceae family abundances HF+GOS group. Overall, increment bifidobacterial population raise fed alone, variation microbiome diversity intervention.
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