Immunomodulatory activity accompanying chicken egg yolk immunoglobulin Y

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Sequential separation of immunoglobulin Y and phosvitin from chicken egg yolk without using organic solvents.

A study was conducted to develop a simple sequential separation protocol to separate phosvitin and IgY from egg yolk without using organic solvents. Egg yolk was diluted with 2 volumes of distilled water (DW), homogenized, and centrifuged. The precipitant was collected and homogenized with 4 volumes of 10% NaCl (wt/vol) in 0.05 N NaOH solution to extract phosvitin. The pH of the homogenate was ...

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Chicken egg yolk immunoglobulin (IgY) is a superior alternative to mammalian immunoglobulin. However, the practical application of IgY in research, diagnostics, and functional food is limited due to complex or time-consuming purification procedures. The objective of this study was to develop a simple, safe, large-scale separation method for IgY from egg yolk. Egg yolk was diluted with 6-fold de...

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ژورنال

عنوان ژورنال: Poultry Science

سال: 2012

ISSN: 0032-5791

DOI: 10.3382/ps.2012-02546