Immunological detection assays for recombinant Shiga toxin & Shigella dysenteriae
نویسندگان
چکیده
منابع مشابه
Ionic requirements for entry of Shiga toxin from Shigella dysenteriae 1 into cells.
The ionic requirements for entry of Shiga toxin into cells were examined by measuring inhibition of protein synthesis after short-term incubations with toxin. The sensitivity of Vero cells and HeLa cells to Shiga toxin was strongly dependent on the divalent cation present. Vero cells were most sensitive in the presence of CaCl2 and SrCl2, whereas HeLa cells were equally sensitive in the presenc...
متن کاملEndocytosis from coated pits of Shiga toxin: a glycolipid-binding protein from Shigella dysenteriae 1
Evidence is presented that endocytosis is involved in the transport to the cytosol of the cytotoxin from Shigella dysenteriae 1, Shiga toxin, which acts by removal of a single adenine residue in 28-S ribosomal RNA. Inhibition of endocytosis by ATP depletion of the cells prevented toxin uptake. Exposure of HeLa S3 and Vero cells to toxin at low extracellular pH, where translocation to the cytoso...
متن کاملEndocytosis from Coated Pits of Shiga Toxin: A Glycolipid-binding Protein from Shigella dysenteriae
Evidence is presented that endocytosis is involved in the transport to the cytosol of the cytotoxin from Shigella dysenteriae 1, Shiga toxin, which acts by removal of a single adenine residue in 28-S ribosomal RNA. Inhibition of endocytosis by ATP depletion of the cells prevented toxin uptake. Exposure of HeLa $3 and Vero cells to toxin at low extracellular pH, where translocation to the cytoso...
متن کاملPurification and biological characterization of shiga toxin from Shigella dysenteriae 1.
Shiga toxin has been purified in milligram quantities to near homogeneity from cell lysates of Shigella dysenteriae 1 strain 3818-0. Purification involved an initial ultracentrifugation, ammonium sulfate fractionation, chromatography on DEAE-cellulose and carboxymethyl cellulose, gel filtration, and preparative isoelectric focusing in sucrose gradients. The purified toxin was resolved by discon...
متن کاملBiological properties of Shigella flexneri 2A toxin and its serological relationship to Shigella dysenteriae 1 toxin.
A toxin extracted from heat-inactivated, alkaline-treated Shigella flexneri 2a showed biological properties similar to those of Shigella dysenteria 1 toxin. The S, flexneri 2a toxin was lethal to mice, enterotoxic for ileal loops of rabbits, and cytotoxic for HeLa cells. A serological relationship between S. flexneri 2a and S. dysenteriae 1 toxin was shown with cross neutralization tests.
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ژورنال
عنوان ژورنال: Indian Journal of Medical Research
سال: 2019
ISSN: 0971-5916
DOI: 10.4103/ijmr.ijmr_308_17