Immunogenic proteins of Trichomonas vaginalis as demonstrated by the immunoblot technique

Immunogenic and Plasminogen-Binding Surface-Associated -Enolase of Trichomonas vaginalis

Identification of Antigenic Proteins in Trichomonas vaginalis

Trichomoniasis is a sexually transmitted disease due to infection with Trichomonas vaginalis, and it can cause serious consequences for women's health. To study the virulence factors of this pathogen, T. vaginalis surface proteins were investigated using polyclonal antibodies specific to the membrane fractions of T. vaginalis. The T. vaginalis expression library was constructed by cloning the c...

Trichomonas vaginalis

We have investigated the effects of a novel neutrophil-activating factor released by Trichomonas vaginalis (TV-NAF) on neutrophil chemotaxis. TV-NAF was present in the supernatant from 107 T. vaginalis (STV) cultured in 1 ml of serum-free Hanks' balanced salt solution (HBSS) at 37°C for 30 min. With a multichamber chemotactic assay, we found that there were 112 + 15 migrated neutrophils (mean ±...

Host plasma proteins on the surface of pathogenic Trichomonas vaginalis.

Sodium dodecyl sulfate-gel electrophoresis and fluorography and fluorography technology revealed that pathogenic Trichomonas vaginalis was able to acquire numerous loosely associated plasma proteins during incubation in normal human plasma. These proteins were readily removed by repeated washing of the parasite in phosphate-buffered saline. Plasma proteins avidly bound to the surface of T. vagi...

Diagnosis of Trichomonas vaginalis infection by PCR

Background: Trichomonas vaginalis infection is the most prevalent sexually transmitted disease in the world. It causes vaginitis, urethritis and preterm birth. It has been associated with nongonococcal urethritis in men. In this study, a polymerase chain reaction (PCR) targeting the beta-tubulin genes of T. vaginalis was developed for the detection of the organism in both vaginal swab and urine...