Immunofluorescence techniques in retrospective diagnosis of human listeriosis

Immunofluorescence Techniques as an Aid to the Diagnosis of Gonorrhoea.

The technique of staining antigens with a fluorescent dye coupled to specific antibody was first applied to the identification of N. gonorrhoeae by Deacon, Peacock, Freeman, and Harris (1959). From this and further studies they concluded that the fluorescent antibody (F.A.) technique was superior to conventional cultural diagnostic methods and afforded a considerable saving in labour and materi...

Immunofluorescence techniques.

anTiBOdies An antibody is a protein complex produced by B cells that initiates an immune response against a target antigen. The basic organization of an antibody includes two functional domains that, together, resemble the letter Y (Figure 1, left). The Fab (fragment having the antigen binding site) domain makes up the arms of the Y, and at the end of each arm is a variable region responsible f...

Comparison of ELISA and Agar Gel Diffusion Techniques in Diagnosis of Human Hydatidosis.

Epidemiology of human listeriosis.

During the 1980s, investigation of several large epidemics of listeriosis confirmed that transmission of L. monocytogenes in food causes human disease. Progress in laboratory detection and subtyping of the organism has enhanced our ability to compare human and environmental isolates of L. monocytogenes. Transmission by foodborne organisms is now recognized as causing both epidemic and sporadic ...

comparison of the amount of debris extruded apically in two rotary techniques: flexmaster and m2

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