منابع مشابه
Rapid two-dimensional immunoelectrophoresis of human serum proteins.
We describe two versions of a two-dimensional immunoelectrophoretic system and illustrate their use for separating and estimating human serum proteins. Each is sensitive, rapid, and simple to perform, and requires no special apparatus and only 0.1-0.6 ml of antiserum. Both procedures appear to be potentially useful in the clinical laboratory. Time required to obtain a stained pattern varies fro...
متن کاملBinding of radiographic contrast media to serum proteins demonstrated by immunoelectrophoresis and radioautography.
BINDING OF RADIOGRAPHIC CONTRAST MEDIA TO SERUM PROTEINS A clinical and experimental investigation of their adverse effects through influence on active steroid hormone levels. by Staffan Wirell, M.D., Department of Diagnostic Radiology, University Hospital of Umeå, S-901 85 Umeå, Sweden. Summary of thesis based on the following papers: I Wirell S.: Adverse reactions at intravenous cholegraphy w...
متن کاملNonspecific precipitation of serum proteins by sodium lauryl sulfate in agar diffusion and immunoelectrophoresis.
The anionic detergent sodium lauryl sulfate (SLS), in a final concentration of 0.1% and greater, reacted with whole serum in agar diffusion and immunoelectrophoresis to form artifactual precipitin lines. These lines occurred when either Ionagar or agarose was used as the supporting gel and were not affected by the presence of urea and 2-mercaptoethanol. Analytic chemical tests confirmed that th...
متن کاملIsoelectric focusing and crossed immunoelectrophoresis of heme proteins in the Escherichia coli cytoplasmic membrane.
Isoelectric focusing (IEF), agarose electrophoresis, and crossed immunoelectrophoresis (CIE) were used to resolve the heme-containing proteins of the Escherichia coli cytoplasmic membrane after solubilization by Triton X-100. Two bands in IEF stained for heme with pI values of 4.7 and 5.3. One of the bands, with an isoelectric point of pH 5.3, was present only when the cells were grown to late ...
متن کاملCrossed immunoelectrophoresis of human platelet membranes.
Human platelet membranes were prepared by the glycerol-lysis technique of Barber and Jamieson (Barber, A. J., and Jamieson, G. A. (1970) J. Biol. Chem 245, 6357-6365), solubilized in 1% Triton X-100 and subjected to crossed immunoelectrophoresis, in 1% agarose, employing a rabbit anti-human platelet membrane antibody in the second dimension. Approximately 20 immunoprecipitates (numbered 1 to ...
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ژورنال
عنوان ژورنال: Nature
سال: 1963
ISSN: 0028-0836,1476-4687
DOI: 10.1038/199283a0