Immunoelectron-microscopic localization of IgE binding site of mugwort pollen

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Immunoelectron-microscopic localization of IgE binding site of mugwort pollen.

To elucidate the IgE binding site of mugwort (Artemisia vulgaris r.) pollen, pollen grains were frozen and fixed using a cryocut. They were incubated with antibodies according to the following sequence: Sera pool of individuals who showed mugwort-RAST class 3 or 4, biotin-labeled goat anti-human IgE antibody, streptavidin-peroxidase and diaminobenzidine. Then, they were observed under electron ...

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ژورنال

عنوان ژورنال: Journal of Korean Medical Science

سال: 1993

ISSN: 1011-8934,1598-6357

DOI: 10.3346/jkms.1993.8.1.30